Supplementary Figure 7: Constitutive activation of ALK5 in forebrain neurons is not affecting neural stem cell pool and production of committed neurons, but is reducing apoptotic markers and increasing newborn neuron survival in the dentate gyrus.
From: ALK5-dependent TGF-β signaling is a major determinant of late-stage adult neurogenesis
(a) Quantification of Sox2+ cells in the SGZ of the dentate gyrus of 3-month-old ALK5Ctrl and ALK5CA mice (n = 5 mice per group, 5 sections per mouse, P = 0.2514). (b) Experimental design for long-term and short-term BrdU-labeling paradigms. (c) Quantification of the total number of BrdU labeled cells and the percentage of BrdU and DCX double-labeled cells in the dentate gyrus of ALK5Ctrl (n = 7) and ALK5CA (n = 6) mice (3-month-old) 1 day after BrdU administration. Five sections per mouse. (d) A representative confocal image of DAPI staining in the dentate gyrus of 3-month-old ALK5CA mice. Arrow points to an apoptotic cell with a pyknotic nucleus (condensed DAPI staining). Scale bar, 20 μm. (e) Immunoblots of hippocampal lysates from 3-month-old ALK5Ctrl (n = 5) and ALK5CA (n = 4) mice probed with antibodies against Bcl-2, Bax, and NSE. Full-length blots are presented in Supplementary Figure 10. (f) Quantification of BrdU and NeuN double-labeled newborn neurons in the dentate gyrus of 4-month-old ALK5Ctrl and ALK5CA mice 28 days after BrdU administration before and after doxycycline treatment (n = 4 mice per group, 5 sections per mouse). Doxycycline × genotype interaction F(1,12) = 2.783, P = 0.1211; doxycycline treatment F(1,12) = 2.036, P = 0.1791; genotype F(1,12) = 2.134, P = 0.1697; Data are presented as mean ± s.e.m. Student's t-test in (a,c). Two-way ANOVA in (f).
