Supplementary Figure 2: Deficiency of ALK5 is not affecting the neural stem cell pool and production of committed neurons.
From: ALK5-dependent TGF-β signaling is a major determinant of late-stage adult neurogenesis
(a,b) Immunohistochemical detection (a) and quantification (b) of Sox2 labeled cells in the dentate gyrus of 3-month-old ALK5loxP/loxP and ALK5cKO mice (n = 7 mice per group, 5 sections per mouse, P = 0.9023). (c) Quantification of the percentage of BrdU and DCX double-labeled cells in the dentate gyrus from 7-month-old ALK5loxP/loxP and ALK5cKO mice after short-term BrdU labeling (n = 5 mice per group, 5 sections per mouse, P = 0.8043). Experimental design inserted on top. (d) Quantification of total PCNA+ proliferating cells in the dentate gyrus of 3-month-old ALK5loxP/loxP and ALK5cKO mice (n = 7 mice per group, 5 sections per mouse, P = 0.8384) (e) Quantification of the percentage of DCX and PCNA double-labeled cells in the dentate gyrus of 3-month-old ALK5loxP/loxP and ALK5cKO mice (n = 7 mice per group, 5 sections per mouse, P = 0.3450). Scale bar is 100 μm. Data are presented as mean ± s.e.m. Student's t-test.
