Supplementary Figure 2: The effects of BK channel shRNAs on nBK channel expression and nuclear Ca2+ signal.
From: Nuclear BK channels regulate gene expression via the control of nuclear calcium signaling
(a) BK channel expression after knockdown by two pairs of lentiviral shRNAs. (b) Paxilline-induced changes in Fluo-4 AM fluorescence intensity in the isolated nuclei after knockdown of BK channel (n = 25 nuclei for each group, unpaired t-test, P = 1.83 × 10−7, t48 = 6.09). (c) Paxilline-induced changes in Fluo-4 dextran fluorescence intensity in the isolated nuclei after knockdown of BK channel (n = 25 nuclei for each group, unpaired t-test, P = 2.67 × 10−7, t48 = 5.98). The bottom and top of the box represent the first and third quartiles, and the band inside the box is the median. The whisker represents the minimum and maximum of all of the data. **P < 0.01. The full-length blots for a are presented in Supplementary Figure 7.
