Supplementary Figure 5: Light responses mediated by cone expression and illumination of Halo57 (K200R W214F) in murine retinitis pigmentosa retinas.
From: Noninvasive optical inhibition with a red-shifted microbial rhodopsin
(a) Comparison of mean spiking for Halo57 (K200R W214F) mutant (n = 16) in ganglion cells downstream from opsin-expressing neurons (9.6 x 1017 photons cm-2 s-1 at 600 nm), using AAV with the mCAR promoter and serotype 8, ∼ 40 days post infection. (b) Confocal fluorescence images of Jaws-GFP (left) and eNpHR3.0-expressing (right) f-RD retinas. Scale bars 20 μm. (c) Retinal ganglion cell spike rates vs. red (left), green (middle), and blue (right) irradiances, comparing Halo57 (K200R W214F) vs. eNpHR. (d) Comparison of retinal ganglion cell spiking under red, green, and blue illumination for eNpHR (n = 21 cells), Halo57 (n = 14 cells), Halo57 (K200R W214F) (n = 16 cells), Jaws (n = 27 cells), ArchT (n = 30 cells) or Mac (n = 13 cells) expressed in mouse cone cells (light intensity was 6.7 x 1017 photons cm-2 s-1 at 470 nm, 1.2 x 1018 photons cm-2 s-1 at 550 nm, and 9.6 x 1017 photons cm-2 s-1 at 600 nm). Values are means ± standard error.
