Supplementary Figure 8: Demonstration of Jaws functionality in awake mouse cortex, using invasive 200-μm fibers.
From: Noninvasive optical inhibition with a red-shifted microbial rhodopsin
(a-b) Gene schematic (a) and corresponding representative glass pipette extracellular recording (b) of different Jaws variants expressed in cortical neurons 6 weeks post-injection in awake mice undergoing red or yellow light illumination (637 or 593 nm, ∼ 130 mW/mm2 out the fiber tip). (c) Light-induced hyperpolarization of a Jaws-expressing neuron patched in the motor cortex of an anesthetized mouse (AAV8-CAG-Jaws; 635 nm; ∼ 130 mW/mm2 out the fiber tip terminating ∼ 500 μm above the electrode tip). (d) Comparison of different Jaws variants 1-3 months post-injection, as measured by suppression of spontaneous firing, change in firing 5-seconds post-illumination, inhibition latency, and recovery latency (n = 14 units for AAV8-CaMKII-Jaws, n = 17 units for AAV8-hSyn-Jaws, n = 6 units for AAV8-hSyn-Jaws-ER2). (e) Spike rasters recorded from a representative neuron (top), and population average (bottom; n = 31 units) of instantaneous firing rate in neurons showing any degree of light-induced suppression, recorded in awake headfixed mice 4-8 weeks after injection of AAV8 encoding Jaws under either the CaMKII (n = 14 units) or synapsin promoter (n = 17 units; black line, mean; grey lines, mean ± s.e.). Values are means ± standard error.
