Supplementary Figure 9: Ex vivo characterization of Jaws-expressing dentate granule cells in acute hippocampal slice.
From: Noninvasive optical inhibition with a red-shifted microbial rhodopsin
(a) Epifluorescence image from Jaws-GFP expressing hippocampus, 4 weeks post-injection. Blue indicates DAPI staining, green indicates GFP fluorescence. Scale bar, 1 mm. (b) Physiological properties upon opsin expression at 4 weeks post-injection in dentate granule cells, including cell input resistance, resting potential, electrically evoked action potential threshold, electrically evoked action potential amplitude. (c) Physiological properties for Jaws-expressing and wildtype dentate granule cells upon red light illumination, including peak and steady-state photocurrent, peak and steady-state photopotential, and post-illumination rebound voltages. n = 12 for Jaws-positive cells, n = 15 for wildtype cells, throughout this panel. Illumination was 1 second at 68 mW/mm2, 625 nm. Values are means ± standard error. Statistics for panel b: * P < 0.05. Panel b was a Student's t-test.
