Supplementary Figure 2: Characterization of cruxhalorhodopsin class halorhodopsins.
From: Noninvasive optical inhibition with a red-shifted microbial rhodopsin
(a) Cruxhalorhodopsin phylogeny tree. Scale bar indicates number of amino acid substitutions per site. (b) Members of the cruxhalorhodopsin class have uniquely red-shifted spectra compared to the N. pharaonis halorhodopsin (halo/NpHR), as reflected by red-green photocurrent ratios in cultured cortical neurons. Regressed lines are shown for each opsin, indicating distinct color shifts. (5 mW/mm2 at 543 or 632 nm). (c) Photocurrents of cruxhalorhodopsin class members as a function of red light irradiance (632 nm; n = 6 cells for each opsin). Values are means ± standard error. All measurements were taken in primary hippocampal neuron culture.
