Supplementary Figure 3: Only when combined, sub-threshold concentrations of Sal003 and DHPG increased eIF2α phosphorylation in WT slices.
From: Translational control of mGluR-dependent long-term depression and object-place learning by eIF2α
Separate applications of low concentrations of DHPG (10 μM, 5 min) or Sal003 (5 μM, 10 min) failed to induce eIF2α phosphorylation; but when at the same low concentrations Sal003 (5 μM, 10 min) immediately followed by DHPG (10 mM, 5 min) the phosphorylation of eIF2α was sharply boosted (n=4 independent experiments, F(3,20)=3.15, p=0.048). Statistical significance was assessed by a one-way ANOVA. Results are displayed as mean ± SEM.
