Supplementary Figure 4: At a concentration that triggers phosphorylation of eIF2α, Sal003 elicited a sustained LTD in WT slices that was insensitive to mGluR1 and mGluR5 antagonists.
From: Translational control of mGluR-dependent long-term depression and object-place learning by eIF2α
a) Brief application of Sal003 (20μM, 10 min) leads to a persistent long-lasting depression of evoked EPSCs (n=6 cells from 4 mice, t=7.07, p=0.0008, paired two sided t-test). b-c) The concurrent application (15min) of the mGluR1 blocker LY367385 (100mM) and the mGluR5 blocker MPEP (10 mM) prevents DHPG-induced LTD (b; n=6 cells from 2 mice, t=7.06,p=3.5X10-5, unpaired t-test) but had no effect on Sal003-induced LTD (c cells from 3 mice; n=8, t=0.53, p=0.61, unpaired two sided t-test). Calibrations: 10 ms, 40 pA. Results are displayed as mean ± SEM.
