Supplementary Figure 9: Spatial recognition increased eIF2α phosphorylation and OPHN1 levels in the hippocampus and the exploratory behavior was similar in all experimental groups.
From: Translational control of mGluR-dependent long-term depression and object-place learning by eIF2α
a,c) Western blots from hippocampal tissue show increased eIF2α phosphorylation (a) and OPHN1 levels (c) after exposure to two objects on day 2 (n=3 mice). b,d) Quantification of normalized p-eIF2α (b, at 10 min t=2.91, p=0.033; at 90 min t=3.37, p=0.020) and OPHN1 (d, at 10 min t=0.287, p=0.79; at 90 min t=2.98, p=0.031). e-g) Raw data for individual animals during training (day 2) or testing (day 3). Plot shows exploration times for individual Eif2s1S/S and Eif2s1S/A mice (e), vehicle-injected and ISRIB-injected mice (f) and control-shRNA-injected and OPHN1-shRNA-injected mice (g) on Day 2 and 3. Statistical significance was assessed by unpaired Student's t-test. Results are displayed as mean ± SEM.
