Supplementary Figure 1: Replication of neuropathology-associated ANK1 hypermethylation in the cortex.
From: Methylomic profiling implicates cortical deregulation of ANK1 in Alzheimer's disease
Bisulfite-pyrosequencing was used to analyze methylation status across eight CpG sites spanning an extended ANK1 region in the Oxford cohort (N = 62). Methylation betas were adjusted for age and gender, and linear models used to compare between controls (Braak score 0-II) and subjects with AD (Braak score V-VI). Average DNA methylation across this region was significantly elevated in all three cortical regions (EC P = 0.0004; STG P = 0.0008; PFC P = 0.014) in affected individuals, most notably in the EC where six of the eight CpG sites assessed are characterized by significant AD-associated hypermethylation (see Fig. 2d). Source Data is provided in the Supplementary Figure Source Data file.
