Supplementary Figure 3: Additional functional testing of enhancers with distinct transcription factor binding and chromatin features

(a) Functional testing of distinct classes of putative enhancers using the Nptx2 reporter. *p = 7.54X10–8 Student’s t-test, two-tailed; Comparison between the average fold induction of luciferase of (FOS + CBP + Increasing H3K27ac) enhancers and FOS only enhancers. **p = 0.0003 Student’s t-test, two-tailed; Comparison between (FOS + CBP + Increasing H3K27ac) enhancers and (FOS + Increasing H3K27ac) enhancers. ***p = 1.69X10–7 Student’s t-test, two-tailed; Comparison between (FOS + CBP + Increasing H3K27ac) enhancers and (FOS + CBP) enhancers.