Supplementary Figure 2: Effects of a Pak1 inhibitor on DMSO-induced increases in pErk expression within the basolateral amygdala
From: Social learning and amygdala disruptions in Nf1 mice are rescued by blocking p21-activated kinase
(a)Bilateral injections of 100% DMSO + the inactive enantiomer PIR3.5 (a pharmacological control for IPA3, 100 μM/100 nl) into the BLA of WT mice induced a robust increase in the no. of local pERK-ir cells, which was attenuated in WT mice that were injected with 100% DMSO + the PAK1 inhibitor IPA3 (100 μM/100 nl: F2,11 = 22.6, p < 0.001, n = 5,3,4). (b) Bilateral injections of 100% DMSO + the inactive enantiomer PIR3.5 (a pharmacological control for IPA3, 100 μM/100 nl) into the BLA of WT mice also reduced in the no. of local ADAM22-ir cells, which was attenuated in WT mice that were injected with 100% DMSO + the PAK1 inhibitor IPA3 (100 μM/100 nl: F2,11 = 22.6, p < 0.001, n = 5,3,4). (c) is a plotted linear regression that illustrates that the no. of ADAM22-ir cells in the BLA is inversely correlated with the no. of pERK-ir cells in the BLA in WT mice from experiment in SFig- 1a-b. (d) contains photomicrographs of pERK and ADAM22-immunoreactive cells in the amygdala from each treatment group. Scale bar is 25 μm. (e) is an illustration of a coronal mouse brain section (taken from Franklin & Paxinos stereotaxic atlas of the mouse brain, 3rd edition) indicating the location of the injection sites for each treatment group within the BLA from -1.46 to -1.94 mm bregma with a 0.5mm scale bar at bottom right. Abbreviations: CeA, central amygdala; ec, external capsule; LA, lateral amygdala; opt, optic tract.
