Supplementary Figure 7: Population analysis of all fibers recorded in entire dataset from Trpm8^–/– and Trpm8^+/+ mice

a, From the entire surveyed population, 15.6% (24/154 single units) responded to cooling in Trpm8^+/+ mice but only 5.7% (11/193 single units) in the Trpm8^–/– mice (Chi-squared test P = 0.0024). There was no change in the proportion of heating sensitive afferents in the Trpm8^–/– mouse. b From the four subtypes of C recorded (C-M, C-Mechano; C-MH, C-MechanoHeat; C-MC, C-MechanoCold, C-MHC, C-MechanoHeatCold), there was a significant reduction in the numbers of recorded C-MC (Chi-squared test P = 0.0025) and no C-MHCs were identified, but there was no change in the numbers of in C-M and C-MH in the Trpm8^–/– mouse. c, We recorded 5 major types of A fiber (A-β; A-δ; A-βC; A-δC; A-δH) none of which showed significant changes in numbers in the Trpm8^–/– mouse.