Supplementary Figure 4: Knockdown of α-Adducin in SOD1G93A mice decreases immunoreactivity for phosphorylated Ser436-α-Adducin. | Nature Neuroscience

Supplementary Figure 4: Knockdown of α-Adducin in SOD1G93A mice decreases immunoreactivity for phosphorylated Ser436-α-Adducin.

From: An α2-Na/K ATPase/α-adducin complex in astrocytes triggers non–cell autonomous neurodegeneration

Supplementary Figure 4

Spinal cord from SOD1G93A mice injected intraspinally with lentivirus expressing short hairpin RNAs targeting α-Adducin and encoding GFP (LV-Addi) or the corresponding control U6 (LV-U6) were subjected to immunohistochemistry using GFP and phospho-α-Adducin (red) antibodies. Knockdown of α-Adducin (LV-Addi) led to a decreased in immunoreactivity of phospho-α-Adducin within the GFP-labeled ventral horn as compared to control U6 (LV-U6) injected ventral horn; scale bar 50μm.

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