Supplementary Figure 5: Knockdown of α-Adducin or α2-Na/K ATPase in SOD1G93A mice do not alter gliosis in the spinal cord.
From: An α2-Na/K ATPase/α-adducin complex in astrocytes triggers non–cell autonomous neurodegeneration
Spinal cord from SOD1G93A mice injected intraspinally with lentivirus expressing short hairpin RNAs targeting α-Adducin or α2-Na/K ATPase or the corresponding control U6 virus were subjected to immunohistochemistry using GFP and the GFAP (red) antibodies. Knockdown of α-Adducin (LV-Addi) or α2-Na/K ATPase (LV-ATPi) had little or no effect on the presence or abundance of astrocytes within the GFP-labeled ventral horn; scale bar 50μm.
