Supplementary Figure 8: Intraspinally injection of control lentivirus in SOD1G93A mice had no effect on motor neuron survival.
From: An α2-Na/K ATPase/α-adducin complex in astrocytes triggers non–cell autonomous neurodegeneration
(a) Spinal cord from end stage SOD1G93A mice injected at age 90 days intraspinally with the control lentivirus encoding GFP (LV-U6 SOD1G93A) was subjected to immunohistochemistry at end stage. End stage was defined as a time point at which the animal was unable to upright itself within 30s of placement on its side. Immunohistochemistry with GFP in SOD1G93A lumbar sections revealed delivery of control injected virus (LV-U6) into the ventral horn; scale bar 100μm. Alternating GFP positive sections were subjected to immunohistochemistry using the GFP antibody and the neurofilment-SMi32 antibody (red), a motor neuron marker, or Nissl stained (lower panels) for quantification of surviving motor neurons within GFP-labeled injected ventral horn and contralateral non-injected ventral horn (n≥20 sections per animal); scale bar 50μm. Control LV-U6 SOD1G93A mice (n=3) displayed equivalent degeneration of motor neurons within injected GFP-labeled ventral horn and non-injected contralateral ventral horn. Arrowheads indicate surviving motor neurons; quantification shown in (b).
