Supplementary Figure 1: α-Adducin in spinal cord is upregulated in symptomatic SOD1G93A spinal cord within astrocytes.
From: An α2-Na/K ATPase/α-adducin complex in astrocytes triggers non–cell autonomous neurodegeneration
(a) Immunoblots for α-Adducin protein at 60 and 90 day old SOD1G93A mice show α-Adducin is upregulated at 90 days. (b) Immunohistochemistry with in sections of the lumbar spinal cord from symptomatic SOD1G93A mice displays Ser436-phosphorylated α-Adducin does not co-localize with the motor neuron marker (SMi32). Arrowheads indicate motor neurons; scale bar 50μm. (c) Immunohistochemistry from sections of control wild type lumbar spinal cord displays Ser436-phosphorylated α-Adducin does not co-localize with the motor neuron marker (SMi32); upper panels. Arrowheads indicate motor neurons. Immunohistochemistry from sections of control wild type lumbar spinal cord displays Ser436-phosphorylated α-Adducin co-localize with the astrocyte marker (GFAP) lower panels. Scale bar 50μm. (a) are cropped; full length images are presented in Supplementary Figure 11.
