Supplementary Figure 3: Lentivirial mediated knockdown in vivo predominately target astrocytes.
From: An α2-Na/K ATPase/α-adducin complex in astrocytes triggers non–cell autonomous neurodegeneration
(a) Immunohistochemistry with GFP in sections of the lumbar spinal cord from SOD1G93A mice displaying percent GFP positive astrocytes (GFAP), microglia (Iba1) and motor neurons (SMi32) 30 days post-injection. Arrowheads indicate motor neurons; scale bar 50μm. (b) Quantifications of percent GFP positive cells revealed lentivirus predominately target astrocytes; n=~300 per cell type/three. All data in bar charts show ± s.e.m (***p<0.001; ANOVA). (c) Immunohistochemistry with GFP in sections of the lumbar spinal cord from wild type mice displaying percent GFP positive astrocytes (GFAP), microglia (Iba1) and motor neurons (SMi32) 30 days post-injection. Arrowheads indicate motor neurons; scale bar 50μm. (d) Quantifications of percent GFP positive cells revealed lentivirus predominately target astrocytes; n=~300 per cell type/three. All data in bar charts show ± s.e.m (***p<0.001; unpaired t-test).
