Supplementary Figure 2: L–PGDS protein is not retained in DRG neurons.

a) Representative western blotting analyses of DRG neurons lysates not infected or infected with NRG1 ICD or L–PGDS as control. Only in L–PGDS overexpressing cultures it is possible to detect L–PGDS protein. Actin serves as a loading control. N = 3 different independent experiments. b) Representative western blotting analyses of DRG conditioned media from not infected or L–PGDS infected neurons. 14 days after infection, cultures were grown in the presence of neurobasal media for additional 48 hours after which the media was tested for L–PGDS expression. L–PGDS is released in the conditioned media. N = 3 different independent experiments. For uncropped pictures of western blots, see Supplementary Figure 10. c) Ponceau loading control of the Western blot showed in Figure 3a. N = 3 different independent experiments. d) Ponceau loading control of the Western blot showed in Figure 3b. N = 3 different independent experiments. e) LC–MS/MS analyses show that PGD2 is not present in conditioned media of DRG neurons overexpressing EGFP. Chromatograms are representative of three different experiments. N = 3 conditioned media preparations.