Supplementary Figure 4: In vitro H–PGDS–/–;L–PGDS–/– neurons are hypomyelinated.
From: Prostaglandin D2 synthase/GPR44: a signaling axis in PNS myelination
a–b) Representative electron microscopy analyses of wild–type (a) and H–PGDS–/–;L–PGDS–/– (b) mouse DRG neurons seeded with rat wild–type Schwann cells and maintained in myelinating conditions for 9 days. Null cocultures are hypomyelinated, and when formed myelin is aberrant (arrow). They also present several naked axons (asterisks), unlike control cultures. N = 9 different independent coculture experiments. Bar: 1 μm c) High power images showing the significant difference in myelin sheath thickness in null cocultures as compared to that of wild–type axons of similar caliber. N = 9 different independent coculture experiments. Bar: 500 nm.
