Supplementary Figure 5: Many dendritic processes yielded their own distinct activity patterns as recorded using the dual-axis microscope in awake behaving mice.

(a) Grayscale images are time-averages over 5 min of movie data, acquired by simultaneous Ca²⁺-imaging of visual areas V1 and LM in a head-restrained mouse at liberty to walk or run on a trackball. (b) Neuronal cell processes that displayed Ca²⁺ activity, as extracted computationally by an established approach to sorting cellular Ca²⁺ signals¹⁵, are depicted in color. Dendrites marked with numbers have their corresponding time traces shown in panel c. (c) 20 example traces of Ca²⁺ dynamics in neuronal processes, divided equally between V1 and LM. Blue shaded epochs denote periods of mouse locomotion. Numbers on the traces indicate the corresponding numbered dendrites shown in panel b. Scale bars in a are 100 μm and also apply to b.