Supplementary Figure 4: Alternative factor combinations generate low numbers of tdTomato-, Tuj1-positive neurons.
From: Modeling pain in vitro using nociceptor neurons reprogrammed from fibroblasts
(a) Ngn1 alone produces a small number of tdTomato, Tuj1-positive cells. (b) The BAM factors produce large numbers of Tuj1-positive cells, a few of which are tdTomato-positive. (c) BAM factors and Ngn1 produce tdTomato, Tuj1-positive neurons, but much less efficiently than the 5 factors (Fig. 1). Representative images for each transcription factor combination study were taken from n = 4 wells from 2 separate transductions. Scale bars: 100 µm. (d) Quantification of the number of tdTomato-positive neurons per well.
