Supplementary Figure 5: Removal of any of the five factors disrupts nociceptor formation. | Nature Neuroscience

Supplementary Figure 5: Removal of any of the five factors disrupts nociceptor formation.

From: Modeling pain in vitro using nociceptor neurons reprogrammed from fibroblasts

Supplementary Figure 5

(a) Transduction of MEFs with all 5 factors (Ascl1, Myt1l, Ngn1, Isl2, and Klf7) efficiently produces tdTomato, Tuj1-positive neurons. (b-f) Removal of Ascl1 (b), Myt1l (c), Ngn1 (d), Isl2 (e), or Klf7 (f) dramatically reduces the number of tdTomato, Tuj1-positive neurons. Representative images of each transcription factor drop out were selected from n = 4 wells from 2 separate transductions. Scale bars: 100 µm.

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