Supplementary Figure 6: Trpv1-Cre::tdTomato iNoc do not express a smooth muscle marker and BAM-derived neurons do not express nociceptor markers.

(a,b) Representative images of TrpV1-Cre::tdTomato iNoc (a) revealing lack of staining for smooth muscle actin (SMA), compared to positive-control C2C12 mouse myoblast cells (b). (c-g) BAM-factor derived, non-subtype specific induced neurons express the pan-neuronal marker Tuj1 (c), but not the nociceptor-specific markers TrpV1 (d), Prph (e), CGRP (f) or NF200 (g). Representative image for SMA in induced nociceptors was selected from n=4 wells from 2 independent transductions and representative image for SMA in myoblasts was selected from n = 4 wells from 1 plating of myoblasts. Representative images for BAM-derived neurons were selected from immunostaining of n = 6 wells from 3 independent transductions. Scale bars: 100 µm.