Supplementary Figure 9: NeuroD1 inhibits the transdifferentiation of human fibroblasts to nociceptors.
From: Modeling pain in vitro using nociceptor neurons reprogrammed from fibroblasts
(a,b) Representative low-mag images of human iNoc derived with 5 factors (without NeuroD1), stained with Tuj1. (c,d) Representative low-mag images of human iNoc derived with 6 factors (including NeuroD1), stained with Tuj1. Representative images for human induced neurons with or without NeuroD1 were selected from immunostaining that was repeated in n = 6 wells from one transduction. Scale bars: 500 µm. The reprogramming efficiency was greater without NeuroD1 than with NeuroD1 (20.7 ± 1.4 cells per field without NeuroD1; 9.7 ± 1.1 cells per field with NeuroD1, n = 6 wells/group; t-test p = 1.0×10−4).
