Supplementary Figure 3: Single transcription factor dropouts from 12 factors identify inhibitory and essential factors.
From: Modeling pain in vitro using nociceptor neurons reprogrammed from fibroblasts
(a) Retroviral transduction of MEFs with a combination of 12 transcription factors produces a small number of tdTomato-positive, Tuj1-positive neurons. (b,c) Removing Runx1 (b), or Brn3a (c) from the 12 factors increases the number of tdTomato-positive cells. (d-f) Removing Ascl1 (d), Myt1l (e), or Klf7 (f) results in a decrease of tdTomato-positive cells. Representative images for each transcription factor dropout were taken from n = 4 wells from 2 separate transductions. Scale bars: 100 µm.
