Supplementary Figure 4: Directed generation of sensory neurons after virally induced NGN2 expression in hES cell–derived NC cells.
From: PIEZO2 is required for mechanotransduction in human stem cell–derived touch receptors
(a-b) Immunocytochemistry for ISL1 (upper panel) and ISL1 and DAPI (lower panel) in hESC-derived NC cells with or without NGN2 virus treatment. Stainings were performed after 4div and 2 days after NGN2 induction. (c-d) NGN2 infected hNC cultures showed an increase in the number of MAFA (upper panel) and NF200 (lower panel) positive neurons after 21 days of differentiation (d), compared to non-infected cells (c). Scale bar 50μm (b, d).
