Supplementary Figure 1: Characterization of hES cell–derived neural crest (NC) cells. | Nature Neuroscience

Supplementary Figure 1: Characterization of hES cell–derived neural crest (NC) cells.

From: PIEZO2 is required for mechanotransduction in human stem cell–derived touch receptors

Supplementary Figure 1

(a) qRT-PCR for known NC markers SOX9, SOX10, PAX3 and P75 and (b) early sensory fate markers BRN3A, NGN1 and NGN2. Lysates prepared from one culture dish of hESC-derived NC cells and processed in triplicates. Fold increase is shown relative to undifferentiated hESCs. Mean ± s.d. (c) Immunocytochmistry of hES-cell-derived neural crest cells with anti-sera against the indicated proteins P75, HNK1, BRN3A. Scale bar, 20μm.

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