Supplementary Figure 8: Tuning curves with dense homogenous sampling and following 24 h.

a. Ten examples of single-cells’ tuning curves, evaluated by presentation of 40 different tones spanning 4 octaves (300 Hz to 4800 Hz) with equal spacing in logarithmic scale, and each tone is presented 10 times for 200ms in pseudorandom order.

Shown is the firing rate, either in a colormap (bottom) or on the y-axis, as a function of distance (%) from the CS+ of the previous day.

From top to bottom: the further the PS is from the CS+ of the previous day, the wider the tuning.

Hence, even with dense homogenous sampling, the characterization of tuning curve show the same attribute of width as a function of distance from the CS+. Moreover, this is evident even in the following day before a new session begins, indicating correlates of overnight memory.

b. Correlation between the width of the tuning curves (in octaves) and the distance between the neuron’s PS and the CS+ from the previous day (solid line, p<0.01, r=0.5). As control, we repeated this analysis using random tones as the CS+ (1000 repetitions, dashed line, mean ± SEM).

c. Distribution of PS was homogenous.