Supplementary Figure 3: Mdivi-1 treatment induces tubular mitochondrial morphology.
From: Mitochondrial control by DRP1 in brain tumor initiating cells
a. Immunofluorescent staining of the mitochondrial marker TOM20 in 387 and 3565 BTICs treated with DMSO or Mdivi-1. Data are represented as mean ± s.e.m. (387: fragmented, p = 0.0032; tubular, p = 0.0010. 3565: fragmented, p = 0.0092; tubular, p = 0.0015 by Student’s t-test; n = 3). b. Analysis of tumor mitochondrial morphology treated in vivo with Mdivi-1. 16 days after tumor implantation, immunocompromised mice bearing orthotopic 387 BTICs (3 × 105 cells/animal) were treated with Midivi-1 (2.5 mg/kg) or DMSO vehicle control. Mitochondria morphology of brain tumor tissue was assessed by Transmission electron microscopy. At least 30 mitochondria were analyzed per experiment. Scale bars indicate 1 µm. Data are presented as mean ± s.e.m. (p = 0.0353 by Student’s t-test; n = 3).
