Supplementary Figure 5: Re-expression of LRP1 and GLUT1 in the hippocampus of Slc2a1+/–APPSw/0 mice using adenovirus-mediated transfer with Ad.mLRP1 and Ad.Slc2a1.
From: GLUT1 reductions exacerbate Alzheimer's disease vasculo-neuronal dysfunction and degeneration
(a) Representative immunoblotting analysis of LRP-515 and mLRP1 levels in 10-month old Slc2a1+/−APPSw/0 mouse hippocampus injected with Ad.Ctrl and Ad.mLRP1. (b) Representative confocal microscopy analysis of LRP1 (green) and lectin-positive (red) microvessels in 10-month old Slc2a1+/−APPSw/0 mouse hippocampus injected with Ad.Ctrl and Ad.mLRP1. Co-localization (yellow) denotes vascular LRP1. Scale bar, 20 µm. (c) Quantification of microvascular LRP1-positive area in 10-month old Slc2a1+/−APPSw/0 mice after hippocampal injection with Ad.Ctrl and Ad.mLRP1. Mean ± SEM, n=3 mice; *p<0.05. (d) Representative confocal microscopy analysis of GLUT1 (green) and lectin-positive (red) microvessels in 10-month old Slc2a1+/−APPSw/0 mouse hippocampus injected with Ad.Ctrl and Ad.Slc2a1. Co-localization (yellow) denotes vascular GLUT1. Scale bar, 20 µm. (e) Quantification of microvascular GLUT1-positive microvessels in 10-month old Slc2a1+/−APPSw/0 mice after hippocampal injection with Ad.Ctrl and Ad.Slc2a1. Mean ± SEM, n=3 mice; **p<0.01. (f) Representative confocal microscopy analysis of LRP1 (green) and lectin-positive (red) microvessels in 10-month old Slc2a1+/−APPSw/0 mouse hippocampus injected with Ad.Ctrl and Ad.Slc2a1. Co-localization (yellow) denotes vascular LRP1. Scale bar, 20 µm. (g) Quantification of microvascular LRP1-positive microvessels in 10-month old Slc2a1+/−APPSw/0 mice after hippocampal injection with Ad.Ctrl and Ad.Slc2a1. Mean ± SEM, n=3 mice; *p<0.05.
