Supplementary Figure 9: Slc2a1 haplosufficiency does not affect red blood cell (RBC) indices and hemoglobin (Hb) oxygen saturation or dissociation in Slc2a1+/–, Slc2a1+/+APPSw/0 and Slc2a1+/–APPSw/0 mice.
From: GLUT1 reductions exacerbate Alzheimer's disease vasculo-neuronal dysfunction and degeneration
(a) Quantification of Glut1 membrane protein levels in circulating RBCs from 2 month old Slc2a1+/− mice. (b) Representative bright field microscopy showing RBC morphology in 2 month old Slc2a1+/+, Slc2a1+/−, Slc2a1+/+APPSw/0 and Slc2a1+/−APPSw/0 mice. (c) Graph showing RBC deformability profiles, a measure of mechanical properties, in 2 month old Slc2a1+/+ (green), Slc2a1+/− (blue), Slc2a1+/+APPSw/0 (red) and Slc2a1+/−APPSw/0 (purple) mice. (d-j) Quantification of RBC hemoglobin (d), mean corpuscular volume (e), mean corpuscular hemoglobin (MCH) (f), mean corpuscular hemoglobin concentration (MCHC) (g), red cell distribution width (RDW) (h), mean arterial oxygen saturation (SaO2) (i), and mean P50 value of oxygen dissociation (j), in 2 month old Slc2a1+/+, Slc2a1+/−, Slc2a1+/+APPSw/0 and Slc2a1+/−APPSw/0 mice. Mean ± SEM, n=3-4 mice per group. ns, nonsignificant.
