Supplementary Figure 4: mRNA for NPY and its receptors are unaltered following 3-cycle DID.
From: NPY signaling inhibits extended amygdala CRF neurons to suppress binge alcohol drinking
(a) Mice that underwent 3-cycle EtOH DID consistently consumed an average of 6.4−7.2 g/kg ethanol and achieved blood ethanol contents (BECs) of approximately 162 mg/dl (N = 9). (b−d) mRNA content of NPY (b; N’s = 10), Y1R (c; N’s = 10), and Y2R (d; CON n = 8, EtOH n = 9) in the BNST was not different between ethanol drinkers and controls one day following 3-cycle DID (unpaired t-tests: p’s > 0.65). All data are presented as mean ± SEM.
