Supplementary Figure 5: Electrophoretic mobility shift assay for the interaction between DISC1 and ITPR1-3'UTR RNA. | Nature Neuroscience

Supplementary Figure 5: Electrophoretic mobility shift assay for the interaction between DISC1 and ITPR1-3'UTR RNA.

From: Disrupted-in-schizophrenia 1 regulates transport of ITPR1 mRNA for synaptic plasticity

Supplementary Figure 5

(a) RNA EMSA assay was performed by incubating biotin-labeled ITPR1 3' UTR RNA with purified GST-hDISC1-FL, GST-hDISC1-CH or GST recombinant protein. (b,c) Dissociation constant of DISC1 for ITPR1 3' UTR RNA. The relative amount of the bound RNA was plotted against the protein concentration. The dissociation constant was calculated as half-maximum binding.

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