Supplementary Figure 2: Subcellular localization of DISC1 in dendrites.
From: Disrupted-in-schizophrenia 1 regulates transport of ITPR1 mRNA for synaptic plasticity
(a,b) DISC1 and MAP2 staining in hippocampal CA1 pyramidal neurons of a mouse brain slice (a) and in cultured hippocampal neurons (b). Scale bar, 10 μm (5 mm in magnified images). (c–i) Staining is shown for DISC1 and GFP-rStaufen (c), SYNCRIP (d), FRMP (e), eIF4G (f), Venus–ITPR1 3' UTR (g), HZF (h) or Ago2/EIF2C2 (i). Scale bars, 20 μm. Higher-magnification images of the boxed areas are shown in the lower right panels, in which asterisks and arrowheads indicate larger and smaller granules in dendrites, respectively. Scale bars in the magnified images represent 5 μm. (j,k) Immunoelectron microscopic localization of DISC1 in CA1 pyramidal neurons of an adult mouse brain. DISC1 immunoreactivity (IR) was detected at neighboring sites of the spine and along microtubules. The arrowheads and arrows represent postsynaptic density and IR of DISC1, respectively. D, dendritic shaft; Pre, presynapse; M, mitochondria. Scale bars represent 500 nm (j) and 1 μm (k).
