Supplementary Figure 3: Effects of neonatal hypoxia on MBP and axonal myelination at P30.
From: GABAergic regulation of cerebellar NG2 cell development is altered in perinatal white matter injury
(a) Representative western blot analysis of MBP and NF200 from P11 and P30 cerebellar lysates. Actin was used as a loading control. (b) Pooled data from 3 independent experiments (each from a single mouse) showed a decrease at P11 in the MBP to NF200 ratio following hypoxia (t3.24 = 3.96, P = 0.03; unpaired Welch two-sample t test). At P30 the MBP to NF200 ratio was not significantly different following hypoxia (t1.23 = 3.65, P = 0.29). (c) Electron microscopy images from P30 cerebellar white matter. (d) Left, scatter plot of g ratios of individual axons versus axon diameter; pooled data from 3 normoxic and 3 hypoxic mice (blue and red, respectively). Fitted lines are linear regressions and shaded areas indicate 95% confidence bands. Right, plot of normalized cumulative probability of g ratio for the 3 normoxic mice (blue dashed lines) and the 3 hypoxic mice (red dashed lines). Solid lines are the averaged probability plots and the shaded areas denote the s.e.m. Hypoxia increased the g ratio from 0.80 ± 0.007 to 0.85 ± 0.005 (t5.1 = 3.77 P = 0.0084).
