Supplementary Figure 4: Western blot analysis shows loss of CNPase and MBP following neonatal hypoxia.
From: GABAergic regulation of cerebellar NG2 cell development is altered in perinatal white matter injury
(a) Western blot analysis of myelin markers CNPase and MBP in whole cerebellum from normoxic and hypoxic animals (P7-P30). Full membranes were cut to probe for both CNPase (210 kDa-40 kDa) and MBP (40 kDa-12 kDa) (dashed line). The same blots were then re-probed for the loading control actin (210 kDa-40 kDa). (b) Pooled data from 3 independent experiments (3 animals) at each age showed significant decreases at all ages in the density of CNPase and MBP (relative to actin). For CNPase at P7 t2.38 = 6.29, P = 0.016; at P11 t2.14 = 9.03, P = 0.0097; at P15 t3.07 = 6.06, P = 0.0084; at P30 t3.18 = 4.66, P = 0.016. For MBP at P7 t2.10 = 5.56, P = 0.028; at P11 t2.68 = 22.31, P = 0.0004; at P15 t3.48 = 14.46, P = 0.0003; at P30 t3.80 = 8.81, P = 0.0011. Graphs show mean ± s.e.m., * P < 0.05, ** P < 0.01, *** P < 0.001.
