Supplementary Figure 6: Neonatal hypoxia reduces interneuron proliferation in the molecular layer, internal granule cell layer and white matter.
From: GABAergic regulation of cerebellar NG2 cell development is altered in perinatal white matter injury
(a) Timeline showing the experimental design for BrdU injections. (b) Representative confocal images of sagittal cerebellar sections from normoxic and hypoxic GAD65-GFP mice at P11. The sections (obtained 72 hrs following BrdU pulse) contain BrdU+ cells (red) in the molecular layer (ML) and internal granule cell layer (IGL). Overlay images of GAD65 (green) and BrdU+ (red) reveal proliferating interneurons (yellow) (white arrowheads). Scale bar 50 μm. (c) Same as b, but for cerebellar white matter. (d) Pooled data. Following hypoxic treatment the number of proliferating interneurons was reduced in the molecular layer (t5.96 = 5.01 P = 0.0019) and internal granule cell layer (t6.14 = 7.77, P = 0.00030). (e) Pooled data. Following hypoxic treatment the number of proliferating interneurons was reduced in the white matter (t2.61 = 5.3 P = 0.045). All tests Welch two-sample unpaired t tests, n = 6 normoxic- and 6 hypoxic mice. Graphs show mean ± s.e.m., * P < 0.05, ** P < 0.01, *** P < 0.001.
