Supplementary Figure 7: Effects of TTX and hyperosmotic sucrose on IPSC frequency in NG2-cells.
From: GABAergic regulation of cerebellar NG2 cell development is altered in perinatal white matter injury
(a) Raster plot illustrating mIPSCs recorded from an NG2-cell in a P7 NG2DsRed mouse and the increase in their frequency in response to application of hyperosmotic sucrose (500 mM; gray bar). Records i-iii in the lower panel illustrate representative mIPSCs recorded at +30 mV from the time periods indicated by the red bars. Similar results were obtained in four cells from three mice; sucrose application increased the mIPSC frequency from 0.059 ± 0.025 Hz to 0.56 ± 0.012 Hz (t3 = 3.97, P = 0.028, Welch two-sample paired t test). (b, c) Pooled data showing the effect of TTX on 100 μM carbachol-induced increases in IPSC frequency. TTX both prevented (b) and reversed (c) the action of carbachol. Symbols in gray denote data that has either been described in the main text or, in the case of c, is a subset of data already shown (Fig. 5a).
