Supplementary Figure 10: Treatment with vigabatrin and tiagabine following neonatal hypoxia reverses NG2-cell proliferation and loss of mature oligodendrocytes.
From: GABAergic regulation of cerebellar NG2 cell development is altered in perinatal white matter injury
(a-c) Representative confocal images of sagittal sections from NG2DsRed mice at P15 following hypoxia (P3–11) and subsequent treatment (P11–15) with vehicle (a), the GABA-T inhibitor vigabatrin (100 mg/kg; b) or the GAT-1 inhibitor tiagabine (50 mg/kg; c). NG2- cells (red) were co-labeled with Olig2 (an oligodendrocyte lineage marker; white), Ki67 (a marker of proliferation; white), CC1 (a marker of mature oligodendrocytes; green) and DAPI (blue). White boxes illustrate the approximate regions in which cells were counted (see Methods and Fig. 7). Scale bars 100 μm.
