Supplementary Figure 5: Regulation of dendritogenesis by GFP-Ube3a1 and GFP-Ube3a2.
From: A coding-independent function of an alternative Ube3a transcript during neuronal development
a) qPCR analysis of GFP RNA expression in primary cortical neurons that had been nucleofected with the indicated GFP-Ube3a-fusion constructs. Values are presented as 40-ct and are representative for multiple independent experiments. b) Representative images of primary hippocampal neurons (DIV 13 - 18) transfected with the indicated shRNA and Ube3a1 expression constructs. c) Representative images of primary hippocampal neurons (DIV 7 - 10) transfected with the indicated expression vectors and treated with BDNF (40 ng/ml) for 48 hours prior to fixation. Scale bar = 50 μm. d) Quantification of dendrite complexity in neurons transfected with the indicated GFP expression plasmids. Values are presented relative to GFP-only transfected neurons. n=3.
