Supplementary Figure 2: Testing ofGECIquant to measure the size of fluorescent beads. | Nature Neuroscience

Supplementary Figure 2: Testing ofGECIquant to measure the size of fluorescent beads.

From: Ca2+ signaling in astrocytes from Ip3r2−/− mice in brain slices and during startle responses in vivo

Supplementary Figure 2

Fluorescence images of 4 and 1 μm diameter beads (white) (Tetraspeck fluorescent microspheres size kit, Life Technologies catalog number T14792) along with their semi-automated detection images from GECIquant (red). All the beads were reliably detected and the measured areas of the beads in cross section were almost identical to the calculated areas, as shown in the graph.

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