Supplementary Figure 3: Testing ofGECIquant to draw around cellular shapes.
From: Ca2+ signaling in astrocytes from Ip3r2−/− mice in brain slices and during startle responses in vivo
Fluorescence image of bovine pulmonary artery endothelial cells (Molecular Devices Fluocells prepared Slide Set catalog number F36924), which were subjected to semi-automated rendering with GECIquant (yellow). The program faithfully captured much of the complexity and accurately outlined the cells and their shapes, as shown in the black-and-white image and in the line profile graph across a region of the cell.
