Supplementary Figure 8: Studies with GCaMP6fflx mice crossed with GLAST-Cre/ERT2 mice.
From: Ca2+ signaling in astrocytes from Ip3r2−/− mice in brain slices and during startle responses in vivo
a. Cartoon of the experiment, whereby GCaMP6fflx mice (JAX #024105) were crossed with GLAST-Cre/ERT2 mice. The GCaMP6fflx mice were donated to JAX by Dr. Hongkui Zeng (Allen Institute for Brain Sciences). All of the information on the genetics and genotyping is available at JAX by searching online for the mouse identification number, 024105. b. Two representative images of GCaMP6f immuno expression (green) in relation to S100β. GCaMP6f was expressed in ~40% of S100β positive astrocytes and of these ~33% were isolated from other GCaMP6f expressing astrocytes. We exploited this feature to image isolated astrocytes like that shown in c. The traces for astrocyte shown in c are shown on the right.
