Supplementary Figure 9: Average data for Ca2+ fluctuation frequencies for somatic fluctuations as well as fluctuations in processes (waves and microdomains).
From: Ca2+ signaling in astrocytes from Ip3r2−/− mice in brain slices and during startle responses in vivo
We analyzed the frequency of the three types of fluctuations using our original data set (Fig 1 of paper) and for isolated astrocytes from AAV2/5 microinjections (Supplementary Fig. 7) and from GCaMP6fflx mice (Supplementary Fig. 8). The frequencies were not statistically different for any type of fluctuation across these three data sets, indicating that our original data set (Fig. 1) contained an undetectable number of Ca2+ fluctuations from adjacent GCaMP6f expressing astrocytes (see Main text for details).
