Supplementary Figure 4: Abnormal presynaptic nerve terminals in Lmx1a/b-ablated DA neurons.
From: Dopaminergic control of autophagic-lysosomal function implicates Lmx1b in Parkinson's disease
(a) Vesicular monoamine transporter 2 (VMAT2) and Bassoon immunostaining in cLmx1a/bCtrl (cCtrl) and cLmx1a/bDatCre (cDatCre) mice. (b) Dopamine transporter (DAT) and Bassoon immunostaining in cCtrl and cDatCre mice. Representative pictures from n= 3 animals per genotype. Scale bar, 20μm. Merge images result from the combination of the green and red channel, and colocalization maps display colocalizing pixels as a white overlay on the green and red channel merge. (c) Optical densitometry from Bassoon immunostained active zones in the striatum of adult (12 months) cLmx1a/bCtrl (cCtrl) and cLmx1a/bDatCre (cDatCre) mice showing reduced occurrence of synaptic active zones in cDatCre mice. n=3 animals per genotype. Data is shown in Arbitrary Units (AU). (d) Pearson correlation coefficient from VMAT2 and Bassoon immunostained striatal sections of adult (12 months) cLmx1a/bCtrl (cCtrl) and cLmx1a/bDatCre (cDatCre) mice. n=3 animals per genotype. (e) Pearson correlation coefficient from DAT and Bassoon immunostained striatal sections of adult (12 months) cLmx1a/bCtrl (cCtrl) and cLmx1a/bDatCre (cDatCre) mice. n=3 animals per genotype. Both Pearson correlation coefficients reveal poor overlap of Bassoon staining with both dopaminergic markers. All data is represented as mean±sem; Mann Whitney test; *p<0.05.
