Supplementary Figure 1: Light-induced phosphorylation of MNK1 in the SCN

(a) Representative microscopic images of coronal SCN sections immunolabeled for p-MNK1 (at Thr250) are shown. For these experiments, animals were entrained under a 12h/12h LD cycle for 14 d and transferred to DD for 2d. On the 3rd day in DD, mice were exposed to light (55 lx, 15 min) at CT15. Mice were sacrificed 30 min after light. Scale bars: 100 μm. (b) Quantitation of intensities of p-MNK1 labeling. Note that light triggered a modest but significant increase of p-MNK1 expression at CT 15. *P<0.05 vs. No light. (c) Quantitation of the levels of ERK, MNK1 and eIF4E in the SCN over a 24-h period (See Figure 1f for Western blot images). The protein levels are normalized to the level of α-tubulin. SCN-enriched tissue was harvested every 4 h throughout 24 h when mice were in DD. (d) Phosphorylation of ERK, eIF4E and total MNK1/2 in the SCN over a 24-h period. SCN-enriched tissue was harvested every 4 h throughout 24 h when mice were in LD. The blots of total ERK and eIF4E were used as control. Full-length blots/gels are presented in Supplementary Figure 6.