Supplementary Figure 2: Binding of CFI/USP and AML-1 to the HTT promoter assessed by EMSA. | Nature Neuroscience

Supplementary Figure 2: Binding of CFI/USP and AML-1 to the HTT promoter assessed by EMSA.

From: A SNP in the HTT promoter alters NF-κB binding and is a bidirectional genetic modifier of Huntington disease

Supplementary Figure 2

Binding of the CFI/USP and AML-1 to the allele 4 and 5 target sequences was assessed following incubation with ST14A nuclear extract. (a) Incubation of the oligonucleotides spanning the CF1/USP site with nuclear extract produced a series of shifted bands with decreased binding to the allele 4 compared to allele 5 oligonucleotide. The strongest shifted band was eliminated upon addition of unlabeled competitor oligonucleotide indicating that this interaction is specific. (b) Incubation of the oligonucleotides spanning the AML-1 site with the ST14A nuclear extract also produced multiple shifted bands, of which the strongest was abolished in the presence of unlabeled competitor oligonucleotide. Lanes 1–2, no nuclear extract; lanes 3, 5, 7, allele 4 oligonucleotide and lanes 4, 6, 8, allele 5 oligonucleotide incubated with 2 µg, 4 µg and 6 µg ST14A nuclear extract, respectively. Lanes 9–10, unlabeled competitor oligonucleotide added to labeled oligonucleotide + protein extract.

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