Supplementary Figure 3: Effects of NF-κB knockdown on p53 expression and CAPE on basal HTT transcriptional activity. | Nature Neuroscience

Supplementary Figure 3: Effects of NF-κB knockdown on p53 expression and CAPE on basal HTT transcriptional activity.

From: A SNP in the HTT promoter alters NF-κB binding and is a bidirectional genetic modifier of Huntington disease

Supplementary Figure 3

(a) HEK293 cells were treated with control or NF-κB targeted siRNA. p53 was down-regulated in the siRNA treated group compared to the control (t(12) = 3.46, P = 0.0047, two-tailed unpaired t-test) (n = 7 per group). (b) ST14A cells transfected with construct 4 or 5 were stimulated with CAPE. The untreated construct 5 displayed a significant reduction in transcriptional activity compared to construct 4 (F(3,38) = 29.9, P < 0.0001, one-way ANOVA with Dunnett’s multiple comparison test). Constructs 4 and 5 displayed similar levels in transcriptional activity when the ST14A cells were treated with CAPE in the absence of a NF-κB pathway activating treatment. The data from four independent reporter assay experiments is shown for each panel (n = 12 per group). Data is presented as mean ± s.e.m.

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